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1
Clinical Pathology Associates, Austin, TX, USA.
2
Pathology Research Core, Mayo Clinic, Rochester, MN, USA.
3
Department of Laboratory Medicine and Pathology, Division of Anatomic Pathology, Mayo Clinic, Rochester, MN, USA.
4
Department of Laboratory Medicine and Pathology, Division of Anatomic Pathology, Mayo Clinic, Rochester, MN, USA. [email protected].
1
Clinical Pathology Associates, Austin, TX, USA.
2
Pathology Research Core, Mayo Clinic, Rochester, MN, USA.
3
Department of Laboratory Medicine and Pathology, Division of Anatomic Pathology, Mayo Clinic, Rochester, MN, USA.
4
Department of Laboratory Medicine and Pathology, Division of Anatomic Pathology, Mayo Clinic, Rochester, MN, USA. [email protected].
This study aims to investigate the utility of digital protocols for Ki-67 immunohistochemistry quantitative analysis ("hot spot" method) in the setting of well-differentiated hepatocellular neoplasms. Resection cases of typical hepatic adenomas (HAs) (n = 40), atypical HAs (n = 9), and well-differentiated hepatocellular carcinomas (WD HCCs) (n = 56) were selected. HAs were further classified by immunohistochemistry using antibodies against liver fatty acid binding protein, glutamine synthetase, B-catenin, hepatic serum amyloid A, and C-reactive protein. Ki-67 proliferative index by immunohistochemistry was evaluated in all cases by digital analysis using a modified neuroendocrine tumor "hot spot" protocol. The proliferative rate of HAs (typical, median 1.2% (range 0-7.4%) and atypical, median 1.0% (range 0.3-3%)) was significantly lower than that of WD HCCs (median 4.5%, range 0-49.8%) (P < 0.0001). Only a few (7.5%) of the adenomas (all inflammatory/telangiectatic type) had proliferative rates higher than 4%, compared to most (51%) of HCCs. Ki-67 is a potentially useful adjunct marker in the evaluation of WD hepatocellular neoplasms, as "hot spot" proliferative rates are consistently very low in HAs but vary significantly in WD HCCs.
Tsujikawa H, et al.
Hum Pathol. 2016 Apr;50:24-33. doi: 10.1016/j.humpath.2015.10.014. Epub 2015 Nov 4.
Hum Pathol. 2016.
PMID:
26997435
Xiong W, et al.
Am J Surg Pathol. 2017 Nov;41(11):1466-1472. doi: 10.1097/PAS.0000000000000955.
Am J Surg Pathol. 2017.
PMID:
28914714
Evason KJ, et al.
Hum Pathol. 2013 May;44(5):750-8. doi: 10.1016/j.humpath.2012.07.019. Epub 2012 Oct 16.
Hum Pathol. 2013.
PMID:
23084586
Free PMC article.
Choi WT, et al.
Hum Pathol. 2017 May;63:1-13. doi: 10.1016/j.humpath.2016.12.025. Epub 2017 Jan 11.
Hum Pathol. 2017.
PMID:
28087475
Review.
Stoot JH, et al.
HPB (Oxford). 2010 Oct;12(8):509-22. doi: 10.1111/j.1477-2574.2010.00222.x.
HPB (Oxford). 2010.
PMID:
20887318
Free PMC article.
Review.
Jiang W, et al.
Bioengineered. 2021 Dec;12(1):7941-7949. doi: 10.1080/21655979.2021.1982844.
Bioengineered. 2021.
PMID:
34612781
Free PMC article.
