The .gov means it’s official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. Share Satellite cells assure postnatal skeletal muscle growth and repair. Despite extensive studies, their stem cell character remains largely undefined. Using pulse-chase labelling with BrdU to mark the putative stem cell niche, we identify a subpopulation of label-retaining satellite cells during growth and after injury. Strikingly, some of these cells display selective template-DNA strand segregation during mitosis in the muscle fibre in vivo, as well as in culture independent of their niche, indicating that genomic DNA strands are nonequivalent. Furthermore, we demonstrate that the asymmetric cell-fate determinant Numb segregates selectively to one daughter cell during mitosis and before differentiation, suggesting that Numb is associated with self-renewal. Finally, we show that template DNA cosegregates with Numb in label-retaining cells that express the self-renewal marker Pax7. The cosegregation of 'immortal' template DNA strands and their link with the asymmetry apparatus has important implications for stem cell biology and cancer. Shinin V, et al. Methods Mol Biol. 2009;482:295-317. doi: 10.1007/978-1-59745-060-7_19. Methods Mol Biol. 2009. PMID: 19089364 Rocheteau P, et al. Cell. 2012 Jan 20;148(1-2):112-25. doi: 10.1016/j.cell.2011.11.049. Cell. 2012. PMID: 22265406 Rudnicki MA, et al. Cold Spring Harb Symp Quant Biol. 2008;73:323-31. doi: 10.1101/sqb.2008.73.064. Epub 2009 Mar 27. Cold Spring Harb Symp Quant Biol. 2008. PMID: 19329572 Review. Jory A, et al. Stem Cells. 2009 Nov;27(11):2769-80. doi: 10.1002/stem.220. Stem Cells. 2009. PMID: 19785007 Collins CA. Curr Opin Pharmacol. 2006 Jun;6(3):301-6. doi: 10.1016/j.coph.2006.01.006. Epub 2006 Mar 24. Curr Opin Pharmacol. 2006. PMID: 16563862 Review. Ribieras AJ, et al. Cardiovasc Ther. 2023 May 19;2023:6679390. doi: 10.1155/2023/6679390. eCollection 2023. Cardiovasc Ther. 2023. PMID: 37251271 Free PMC article. Kloc M, et al. Stem Cell Investig. 2023 Apr 13;10:9. doi: 10.21037/sci-2023-009. eCollection 2023. Stem Cell Investig. 2023. PMID: 37077316 Free PMC article. Review. Fu X, et al. Cell Regen. 2022 Dec 2;11(1):40. doi: 10.1186/s13619-022-00142-7. Cell Regen. 2022. PMID: 36456659 Free PMC article. Review. Chen W, et al. Methods Mol Biol. 2023;2587:537-553. doi: 10.1007/978-1-0716-2772-3_29. Methods Mol Biol. 2023. PMID: 36401049 Xu J, et al. J Anim Sci. 2022 Aug 1;100(8):skac001. doi: 10.1093/jas/skac001. J Anim Sci. 2022. PMID: 35908789 Free PMC article.