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RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay
实时监测凋亡进程中的磷脂酰丝氨酸
(PS)
从膜内到膜外的外翻。试剂中提供在早期凋亡时可与外翻到膜外的
PS
结合的
Annexin V
萤光素酶融合蛋白，发光信号检测简单。试剂中还提供
DNA
结合型染料
,
可在膜完整性丧失进入细胞产生荧光信号。
发光和荧光信号的组合检测以及光信号出现的时机，是区分晚期凋亡的继发性坏死和其他毒性事件导致的坏死的关键。
此检测方法无需裂解细胞，直接
“
加样
-
读数
”
，可对一个孔进行多次检测。可实时监测凋亡，而无需处理多块多孔板
,
无需复杂的操作或特殊的检测仪器。只需能够检测发光和荧光的检测仪即可。
不同的剂量，多重的时间点，一个检测平板
当化合物处理来确定化合物效力的时候，使用这种实时检测的试剂，可从同一个孔中反复检测发光和荧光信号，可比使用终点法使用更少的平板和更少的试剂。
试剂兼容多种类型的细胞，可用于处理前或实时检测梯度浓度处理和时间依赖的凋亡进程。
此实验中
, K562
细胞用不同剂量的
bortezomib
处理
16
小时。
上图
:
富集细胞
,
洗涤, 以
Alexa Fluor
®
488 (
绿色荧光
, PtdSer:AnxV
结合
)
和
7-AAD (
红色荧光
,
显示膜完整性
)
，以流式细胞仪分析
(10,000 events).
下图
:
在
RealTime-Glo™ Annexin V Apoptosis Assay reagent
存在下，
K562
细胞
(10,000/
孔
)
以梯度稀释的
bortezomibin
处理。动态监测发光信号
(PtdSer:AnxV
结合
)
和荧光信号
(
膜完整性
)
。显示
16
小时的监测数据。
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 8,598,198 and 9,458,499 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889 and 9,797,890 and other patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 8,598,198 and 9,458,499 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889 and 9,797,890 and other patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889 and 9,797,890 and other patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889 and 9,797,890 and other patents pending.
RealTime-Glo™ MT Cell Viability Assay
A bioluminescent method to kinetically monitor viability in cell culture up to 72 hours.
G9711, G9712, G9713
CellTox™ Green Cytotoxicity Assay
Measures changes in membrane integrity. Kinetically monitors cytotoxicity up to 72 hours with multiplex capability.
G8741, G8742, G8743, G8731
NanoBiT® PPI Starter Systems
用于检测活细胞中蛋白相互作用的互补报告基因技术
N2014, N2015, N2016
CellTiter-Fluor™ Cell Viability Assay
A non-lytic, fluorescent cell viability assay with multiplex capability.
G6080, G6081, G6082
CellTiter-Glo® 2.0 Cell Viability Assay
Updated CellTiter-Glo® Cell Viability Assay with improved reagent stability. Quantifies cell proliferation based on ATP detection.
G9241, G9242, G9243
Caspase-Glo® 9 Assay Systems
Caspase-Glo® 9 Assay是一种均质发光检测试剂盒，检测Caspase-9活性。
G8210, G8211, G8212
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