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Optical sectioning with ZEISS Apotome 3 allows you to efficiently minimize out-of-focus light. Create crisp images and 3D renderings, even of thicker specimen, while your microscope remains just as easy to operate as always. Go one step further with Apotome Plus and achieve confocal-like image quality with your widefield microscope.

Apotome 3 significantly increases the axial resolution compared to conventional fluorescence widefield microscopy: You obtain optical sections that allow 3D rendering, even from thick specimens. Three grids of different geometries give you the best resolution for each objective. You can focus on your experiment as the ideal illumination structure is automatically selected, always resulting in high-contrast optical sections.

_{Caption: Cos7 cells (nuclei stained with Hoechst, tubulin with Alexa 488 and Phalloidin with Alexa 568) imaged with Plan Apochromat 63x/1.4.}

Purely software-based methods require either prior knowledge of the sample (AI based methods) or rely on complex algorithms that have not been peer-reviewed. Users must trust that these black-box solutions do not falsify information when “enhancing” the image. ZEISS Apotome 3 uses the information from the structured illumination combined with documented algorithms to create a crisp optical section you can trust.
^{Caption: Cortical neurons (left: Widefield; right: Apotome 3). Courtesy of L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany.}

Resolve details that were not visible before with your widefield microscope: With Apotome Plus, you can obtain structural information at lateral resolution down to 180 nm. The combination of structured illumination with state-of-the-art image processing substantially improves the signal-to-noise ratio and resolution in x, y, and z.
^{Caption: 35 μm sagittal section of adult mouse brain, imaged with ZEISS Axio Observer and ZEISS Apotome, processed with Apotome Plus. Sample courtesy of University of California, Davis / NIH NeuroMab Facility.}

Your experiments often evolve in complexity and requirements. That’s why you need adaptable equipment. Use Apotome 3 with metal halide lamps, economic white light LEDs, or a gentle, multi-color LED light source of the ZEISS Viluma illumination system. Whether you work with DAPI, Alexa488, Rhodamin, Cy5, or with vital dyes such as GFP or mCherry – Apotome 3 adapts to your fluorophores and light source, creating the sharp and brilliant images you expect.

Apotome 3 uses a grid to generate a pattern of intensity differences. If out-of-focus light is present at a certain region of the sample, the grid becomes invisible. After the fluorescence of a grid position is acquired, the grid moves to the next position. A true optical section with higher contrast and resolution is calculated.

Bovine pulmonal arterial endothelial (BPAE) cells having nuclei stained with DAPI, F-actin with Alexa 488 phalloidin and mitochondria with MitoTracker Red CMXRos. Compared to the widefield image, Apotome removes out of focus light creating a crisp optical section. Apotome Plus further improves image quality and resolution resolving even finer structures.