汤葆莎，吴俐，翁敏劼，等. 杏鲍菇528深层发酵生物合成麦角硫因的培养基优化 [J]. 福建农业学报，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016 引用本文: 汤葆莎，吴俐，翁敏劼，等. 杏鲍菇528深层发酵生物合成麦角硫因的培养基优化 [J]. 福建农业学报，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016 TANG B S, WU L, WENG M J, et al. Optimization of medium for biosynthesis of ergothioneine by submerged fermentation of Pleurotus eryngii 528 [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016 Citation: TANG B S, WU L, WENG M J, et al. Optimization of medium for biosynthesis of ergothioneine by submerged fermentation of Pleurotus eryngii 528 [J]. Fujian Journal of Agricultural Sciences ，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016 汤葆莎，吴俐，翁敏劼，等. 杏鲍菇528深层发酵生物合成麦角硫因的培养基优化 [J]. 福建农业学报，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016 引用本文: 汤葆莎，吴俐，翁敏劼，等. 杏鲍菇528深层发酵生物合成麦角硫因的培养基优化 [J]. 福建农业学报，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016 TANG B S, WU L, WENG M J, et al. Optimization of medium for biosynthesis of ergothioneine by submerged fermentation of Pleurotus eryngii 528 [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016 Citation: TANG B S, WU L, WENG M J, et al. Optimization of medium for biosynthesis of ergothioneine by submerged fermentation of Pleurotus eryngii 528 [J]. Fujian Journal of Agricultural Sciences ，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016 目的 为提高杏鲍菇528发酵物的麦角硫因含量，筛选杏鲍菇528培养的营养配方，以期为开发高含量的麦角硫因杏鲍菇深层液体发酵物提供理论基础。 方法 以固体培养菌丝生长速度和菌丝形态探讨杏鲍菇528菌丝生长的适宜碳氮源；采用单因素试验，以发酵物干重、沉淀物情况和初始pH值为指标，研究杏鲍菇528液体深层发酵菌丝生长的营养配方，探究不同氨基酸对深层液体培养发酵物麦角硫因含量的影响；最后通过L ₉ （3 ⁴ ）正交试验分析提高杏鲍菇528发酵物麦角硫因含量的深层发酵培养基配方。 结果 杏鲍菇528固体培养菌丝生长的适宜碳、氮源分别为玉米糁粉和蛋白胨；在杏鲍菇528液体发酵培养中，玉米糁粉2.0%和蛋白胨2.0%组合碳氮源培养基液体培养的发酵物干重值达33.30±0.81 g·L ⁻¹ ，显著高于其他浓度比例的碳氮源组合，且沉淀物情况和初始pH更适宜杏鲍菇528液体培养；天冬氨酸、半胱氨酸、组氨酸、精氨酸、谷氨酸这5种氨基酸均可明显提高杏鲍菇528发酵物麦角硫因产率，其中组氨酸诱导培养的杏鲍菇液体发酵物中麦角硫因含量最高，是对照组的3.73倍，且明显高于其他氨基酸。 结论 杏鲍菇528深层发酵生物合成麦角硫因的最佳培养基配方为：玉米糁粉2.0%、蛋白胨1.5%、组氨酸0.15%、麦麸细粉1.0%、KH ₂ PO ₄ 0.1%、MgSO ₄ 0.1%、谷氨酸0.1%和维生素B ₆ 0.1%，在此条件下，杏鲍菇发酵物麦角硫因含量达20.50±1.80 mg·L ⁻¹ 。 杏鲍菇528 /  液体发酵 /  麦角硫因 / Abstract: Objective In order to increase the ergothioneine content of Pleurotus eryngii 528 fermented products, the nutritional formula of Pleurotus eryngii 528 was screened to provide a theoretical basis for the development of high content of ergothioneine in submerged fermentation of Pleurotus eryngii . Method The suitable carbon and nitrogen sources for Pleurotus eryngii 528 mycelium growth were discussed based on the solid culture mycelium growth rate and mycelium morphology. A single factor test was conducted to study the nutritional formula of mycelium growth in Pleurotus eryngii 528 submerged fermentation, with the indexes of the dry weight of the fermented product, the sedimentation and the initial pH value of the medium. The effects of different amino acids on ergothioneine content in submerged fermentation broth were investigated. Finally, L ₉ (3 ⁴ ) orthogonal test was used to analyze the formula of submerged fermentation medium for increasing ergothioneine content in Pleurotus eryngii 528. Result Corn grits and peptone were the suitable carbon and nitrogen sources for the growth of Pleurotus eryngii 528. In the liquid fermentation of Pleurotus eryngii 528, the dry weight of the fermentation product in the liquid culture medium of corn grits powder 2.0% and peptone 2.0% reached 33.30±0.81 g·L ⁻¹ , which was significantly higher than that of other carbon and nitrogen source combinations, and the sediment condition and initial pH value were more suitable for Pleurotus eryngii 528 liquid culture. Aspartic acid, cysteine, histidine, arginine and glutamic acid improved significantly the ergothioneine yield of Pleurotus eryngii 528 fermented product. The content of ergothioneine in the liquid fermentation of Pleurotus eryngii induced by histidine was the highest, which was 3.73 times of the control group, and was significantly higher than other amino acids. Conclusion The optimum medium formula of ergothioneine biosynthesis by submerged fermentation of Pleurotus eryngii 528 was as follows: corn grits 2.0%, peptone 1.5%, histidine 0.15%, wheat bran powder 1.0%, KH ₂ PO ₄ 0.1%, MgSO ₄ 0.1%, glutamic acid 0.1% and vitamin B6 0.1%. Under these conditions, the content of ergothioneine in Pleurotus eryngii fermentation reached 20.50±1.80 mg·L ⁻¹ . Key words: Pleurotus eryngii 528 /  liquid fermentation /  ergothioneine /  histidine  配方
Formula玉米糁粉
Corn
grits/%蛋白胨
Peptone/%麦麸细粉
Wheat bran
powder/%谷氨酸
Glutamic
acid/%维生素B ₆
Vitamin
B ₆ /%KH ₂ PO ₄ /%MgSO ₄ /%沉淀物情况
Sediment
conditionpH发酵物干重
Dry weight of fermentation/
(g·L ⁻¹ ) A1.01.01.00.10.10.10.1+4.9624.60±0.17 DB1.02.01.00.10.10.10.1+5.1031.20 ±0.99 CC1.03.01.00.10.10.10.1+5.1131.50 ±1.19 CD2.01.01.00.10.10.10.1++4.9624.70±0.11 DE2.02.01.00.10.10.10.1++5.1033.30±0.81 AF2.03.01.00.10.10.10.1++5.1132.90±1.10 BG3.01.01.00.10.10.10.1+++++4.9524.90±0.25 DH3.02.01.00.10.10.10.1+++++5.1133.10±0.70 ABI3.03.01.00.10.10.10.1+++++5.1232.80±0.64 B 注：字母不同表示差异显著（ P ＜0.05）；+表示沉淀程度：+为沉淀少，++为沉淀较多，+++++沉淀多。
Note: Different letters indicate significant differences ( P ＜0.05); + indicates the degree of precipitation, + means less precipitation, ++ means more precipitation, +++++means the most precipitation. 序列号
Serial
number因素 Factor/%麦角硫因含量
Ergothioneine
content/
(mg·L ⁻¹ )玉米糁粉
Corn grits
A蛋白胨
Peptone
B组氨酸
Histidine
C空列
Null column
D 11.51.50.10110.3921.52.00.15213.6031.52.50.20316.4142.01.50.15319.4852.02.00.20118.4962.02.50.10211.9172.51.50.20219.4182.52.00.1039.9892.52.50.15116.92$ {{\bar K}_1}$13.4716.4310.7615.27$ {{\bar K}_2}$16.6314.0316.6714.97$ {{\bar K}_3}$15.4415.0818.1115.29 R 3.162.407.350.32 变异来源
Source of
variation偏差平方和
Deviation
sum of
squares自由度
Degrees of
freedom F 值
F value显著性
Significance A15.2921.90B8.6721.08C90.95211.30*空列
Null column0.1920.02误差
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