目的
观察壮药海南茄对小鼠的急性毒性,评价其安全性。
方法
确定小鼠出现100%死亡(Dm)和100%存活 ( Dn)的海南茄给药剂量,在Dm和Dn剂量间按1:0.8设定5个剂量组,分别按250,200,160,128,102.4 g·kg
-1
灌胃给药1次,连续观察14 d,观测灌胃给予海南茄水溶液后小鼠毒性反应及死亡率。
结果
给药4 h后,除最低剂量组(102.4 g·kg
-1
)外,其他各组均出现小鼠死亡。250,200,160,128 g·kg
-1
给药组分别死亡10,8,6,3只。海南茄灌胃给药LD
50
为153.02 g· kg
-1
,95%置信区间为(136.55,171.47) g·kg
-1
。
结论
海南茄有一定毒性,临床应用应参考其毒性合理使用。
关键词
:
毒性,急性
半数致死量
Abstract
:
Objective
To evaluate the safety of
Solanumprocumbens
by studying its acute toxicity to mice.
Methods
The dosage of 100% death (Dm) and 100% survive (Dn) were determined. Five groups were set between the dosage of Dm and Dn in a 1:0.8 ratio, and then were intragastrically administrated once at the dosage of 250,200,160,128,102.4 g·kg
-1
respectively.Toxicity and mortality of mice after intragastricly administration of
Solanumprocumbens
were observed for 14 days continuously.
Results
After four hours of administration, there were death in each group except the lowest dosage group(102.4 g·kg
-1
).Number of death of the groups 250,200,160 and 128 g·kg
-1
were 10,8,6 and 3 respectively.LD
50
of
Solanumprocumbens
was 153.02 g·kg
-1
,the 95% confidence interval was (136.55,171.47) g·kg
-1
.
Conclusion
Solanumprocumbens
has a certain toxicity.More attention should be payed to its toxicity for clinical rational drug use.
中草药毒性实验是以暴露中草药固有毒性、揭示毒性性质和程度为目的。开展中草药毒性实验是研制中药新药的基本要求,可为临床安全用药提供科学依据。中草药毒性实验大致分为急性毒性实验、长期毒性实验和特殊毒性实验。急性毒性实验作为全身给药的毒性研究内容之一,也是开展药物安全性评价的第一步。可为长期毒性及特殊毒性实验的剂量选择和指标设定提供参考
[
4
]
。
壮族方药是祖国医药宝库的重要组成部分。除了历代本草典藉对壮族医药有所记载之外,广西史籍或地方县志中也载有壮族特色的药物和用药经验
[
5
]
。
笔者在本实验中对壮药海南茄的水提液进行小鼠的LD
50
测定,为海南茄安全性评价提供参考。实验结果提示,海南茄灌胃给药LD
50
为153.02 g·kg
-1
,以民间用药剂量15 g·(60 kg)
-1
体质量计算,相当于人日拟用剂量的67.26倍,具有一定毒性,临床中应参考其毒性合理使用。
The authors have declared that no competing interests exist.
... 海南茄为广西壮族民间常用的药材,系茄科植物海南茄(
Solanumprocumbens
Lour.)的全株
[
1
]
.在我国主要分布于广西、广东、海南等地,广西主要分布于北海、合浦和防城港等海边地区.民间认为,本品具有清热毒、消肿痛、利咽喉功效.用于咽喉疼痛、乳痈、毒蛇咬伤、跌打损伤的治疗
[
1
]
.文献记载,海南茄“有小毒”
[
2
]
.笔者在本实验中对小鼠灌胃给予海南茄水提取液,观察小鼠毒性反应,并计算海南茄水提取液半数致死量,评价海南茄安全性,以期为新药研发和临床用药提供实验依据. ...
... [
1
].文献记载,海南茄“有小毒”
[
2
]
.笔者在本实验中对小鼠灌胃给予海南茄水提取液,观察小鼠毒性反应,并计算海南茄水提取液半数致死量,评价海南茄安全性,以期为新药研发和临床用药提供实验依据. ...