Department of Chemistry, College of Science for Women, University of Baghdad, Al-Jadriya, Baghdad, Iraq.
Sakarya University, Biomedical, Magnetic and Semiconductor Materials Application and Research Center (BIMAS-RC), 54187, Sakarya, Turkey; Sakarya University, Biomaterials, Energy, Photocatalysis, Enzyme Technology, Nano & Advanced Materials, Additive Manufacturing, Environmental Applications and Sustainability Research & Development Group (BIOEℕAMS R&D Group), 54187, Sakarya, Turkey.
Sakarya University, Biomaterials, Energy, Photocatalysis, Enzyme Technology, Nano & Advanced Materials, Additive Manufacturing, Environmental Applications and Sustainability Research & Development Group (BIOEℕAMS R&D Group), 54187, Sakarya, Turkey; Sakarya University, Science & Arts Faculty, Department of Chemistry, 54187, Sakarya, Turkey.
磁性纳米颗粒（MNP）被透明质酸（HA）修饰。在功能化过程之后，已使用两种不同的策略将异柠檬酸脱氢酶（IDH）固定在MNP上。在第一种策略中，制备了交联的酶聚集体。为此，首先合成透明质酸修饰的磁性纳米颗粒交联的异柠檬酸脱氢酶（IDH / HA / MNPs-CLEAs）细酶聚集体，然后使用牛血清白蛋白（BSA）作为辅助原料合成IDH / BSA / HA / MNPs-CLEAs。在第二种策略中，将IDH有效地固定在HA / MNPs表面。MNP及其衍生物的特征已通过X射线衍射（XRD），扫描电子显微镜（SEM），傅立叶变换红外光谱（FTIR），振动样品磁力计（VSM），和zeta电位测量。IDH在IDH / HA / MNP，IDH / HA / MNPs-CLEA和IDH / BSA / HA / MNPs-CLEA中的活性和稳定性得到增强。此外，固定化的酶很容易通过外磁体从反应介质中分离出来并重复使用多次。获得的发现表明，HA / MNP是IDH的新型结合剂/载体系统，固定在该系统上的IDH可以成为非常重要的生物催化剂，以高精度和高灵敏度用于测定饮用水和其他生物溶液中的镁。
Magnetic nanoparticles (MNPs) were modified by hyaluronic acid (HA). After the process of functionalization, two different strategies have been used to immobilize isocitrate dehydrogenases (IDH) on MNPs. In the first strategy, cross-linked enzyme aggregates were prepared. For this, firstly hyaluronic acid modified magnetic nanoparticles cross-linked enzyme fine aggregates of isocitrate dehydrogenases (IDH/HA/MNPs-CLEAs) were synthesized, and secondly bovine serum albumin (BSA) as co-feeder was used to synthesize the IDH/BSA/HA/MNPs-CLEAs. In the second strategy, the IDH was effectively immobilized on the HA/MNPs surface. The features of MNPs and its derivatives have been studied by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FTIR), vibrating sample magnetometer (VSM), and zeta potential measurements. The activity and stability of IDH in IDH/HA/MNPs, IDH/HA/MNPs-CLEAs, and IDH/BSA/HA/MNPs-CLEAs were enhanced. Besides, the enzyme immobilized was readily separated via external magnet from the reaction medium and reused many times. The acquired findings indicate that HA/MNPs are a novel binder/support system to IDH, and IDH immobilized on this system can become a very important biocatalyst working with high accuracy and sensitivity for the determination of magnesium in drinking water and other biological solutions.
