¹ Department of Tuberculosis Control, Center for Disease Control and Prevention in Wusheng County, Sichuan Province, Guang’an 638401, China
² Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Science, Shanghai Medical College, Fudan University, Shanghai 200032, China

Abstract:

Sputum specimens were collected from 1370 presumptive tuberculosis patients attending to the tuberculosis clinic of Center for Disease Control and Prevention in Wusheng County of Sichuan Province from July 1, 2018 to December 31, 2020. The sputum was cultured using manual liquid culture method and neutralization centrifugal solid culture method at the same time. The positive rate of the manual liquid culture method was 25.8% (353/1370), significantly higher than that of the neutralization centrifugal solid culture method (20.0%, 274/1370) ( χ ² =12.907, P<0.001). The median time to detection was 2 (2, 3) weeks for the manual liquid culture method, which was significantly shorter than that for the solid culture method (3 (2, 4) weeks, Z=-9.491, P<0.001). The manual liquid culture method significantly improved the positive rate, significantly shortened the time to detection, and was comparable in cost to solid culture, making it suitable for application in county-level laboratory.

Key words: Laboratory techniques and procedures, Mycobacterium tuberculosis, Culture techniques

邱勇, 李蒙, 屈榕, 田发君. 县级实验室应用结核分枝杆菌手工液体培养技术检测结果分析[J]. 结核与肺部疾病杂志, 2022, 3(2): 173-176. doi: 10.19983/j.issn.2096-8493.20210136

QIU Yong, LI Meng, QU Rong, TIAN Fa-jun. Analysis of manual liquid culture method in county-level laboratory to detect Mycobacterium tuberculosis[J]. Journal of Tuberculosis and Lung Disease, 2022, 3(2): 173-176. doi: 10.19983/j.issn.2096-8493.20210136

两种培养方法报告阳性时间

报告阳性 时间	手工MFLM液体培养		报告阳性 时间	中和离心固体培养
	报告阳性例数	报告阳性率(%)		报告阳性例数	报告阳性率(%)
0.5周	34	9.6	1周	7	2.6
1周	50	23.8	2周	68	27.4
1.5周	0	23.8	3周	106	66.1
2周	137	62.6	4周	55	86.1
3周	79	85.0	5周	15	91.6
4周	35	94.9	6周	10	95.3
5周	8	97.2	7周	8	98.2
6周	10	100.0	8周	5	100.0
合计	353			274

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