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Proopiomelanocortin (POMC) Precursor
Antibody Production & Evaluation
Image

Protocol for Immunohistochemistry:
Tissue Sample Mouse hippocampus and rat cortex
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-POMC Precursor (27-52) (Porcine) Antiserum (Catalog No.:H-029-30)
Optimal Dilution 1:100 (1hour at RT)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec

Catalog # H-029-30
Standard Size 50 µl
Sequence Trp - Cys - Leu - Glu - Ser - Ser - Gln - Cys - Gln - Asp - Leu - Ser - Thr - Glu - Ser - Asn - Leu - Leu - Ala - Cys - Ile - Arg - Ala - Cys - Lys - Pro

Disulfide Bridge: Cys2 - Cys24 and Cys8 - Cys20
Species Porcine
Host Rabbit
Reconstitution For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage Condition Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content This vial contains 50µl of Rabbit Anti-Proopiomelanocortin (POMC) Precursor (27-52) (Porcine) Serum in the lyophilized form.
Recommended Dilution Factor Western Blot - 1:1000. (J Biol Chem. 2001 Jan 12;276(2):1466-73.)
Immunohistochemistry - 1:5000 (J Clin Endocrinol Metab. 2007 Mar;92(3):1145-54.)
Cross Reactivity not canine
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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Wittmann G, Hrabovszky E, Lechan RM. Distinct glutamatergic and GABAergic subsets of hypothalamic pro-opiomelanocortin neurons revealed by in situ hybridization in male rats and mice.
J Comp Neurol. 2013;521(14):3287-302.

Tavares E, Maldonado R, Minano FJ. Aminoprocalcitonin-mediated suppression of feeding involves the hypothalamic melanocortin system.
Am J Physiol Endocrinol Metab. 2013 Apr 9;

Zhan C, Zhou J, Feng Q, et al. Acute and long-term suppression of feeding behavior by POMC neurons in the brainstem and hypothalamus, respectively.
J Neurosci. 2013;33(8):3624-32.

Praful S. Singru, Gábor Wittmann, Erzsébet Farkas, Györgyi Zséli, Csaba Fekete, and Ronald M. Lechan. Refeeding-Activated Glutamatergic Neurons in the Hypothalamic Paraventricular Nucleus (PVN) Mediate Effects of Melanocortin Signaling in the Nucleus Tractus Solitarius (NTS).
Endocrinology 2012 153: 3804-3814; doi:10.1210/en.2012-1235

Cheng C., Central and Peripheral Administration of Secretin Inhibits Food Intake in Mice through the Activation of the Melanocortin System
Neuropsychopharmacology. 2011 January; 36(2): 459–471. doi:  10.1038/ npp.2010.178

DeBoer et al. Administration of IL-1beta to the 4th ventricle causes anorexia that is blocked by agouti-related peptide and that coincides with activation of tyrosine-hydroxylase neurons in the nucleus of the solitary tract.
Peptides. 2009 Feb;30(2):210-8.

Shen et al. Characterization of apolipoprotein A-IV in brain areas involved in energy homeostasis.
Physiol Behav. 2008 Sep 3;95(1-2):161-7.

Tavares et al. Procalcitonin N-terminal peptide causes catabolic effects via the hypothalamus and prostaglandin-dependent pathways.
Neuroendocrinology. 2008;88(4):316-26.

Tanabe et al. Functional Single-Nucleotide Polymorphisms in the Secretogranin III (SCG3) Gene that Form Secretory  Granules with Appetite-Related Neuropeptides Are Associated with Obesity
J Clin Endocrinol Metab. 2007 Mar;92(3):1145-54.

Cheng et al. Type 1 corticotropin-releasing factor receptors in the ventromedial hypothalamus promote hypoglycemia-induced hormonal counterregulation
Am J Physiol Endocrinol Metab. 2007 Sep;293(3):E705-12.

Claret et al. AMPK is essential for energy homeostasis regulation and glucose sensing by POMC and AgRP neurons
J Clin Invest. 2007 Aug;117(8):2325-36.

Evans et al. Organization of Endogenous Opioids in the Rostral Agranular Insular Cortex of the Rat
J Comp Neurol. 2007 Jan 20;500(3):530-41.

Dudanova et al. Important contribution of alpha-neurexins to Ca2+-triggered exocytosis of secretory granules.
J Neurosci. 2006 Oct 11;26(41):10599-613.

Reyes et al. Pro-opiomelanocortin colocalizes with corticotropin- releasing factor in axon terminals of the noradrenergic nucleus locus coeruleus.
Eur J Neurosci. 2006 Apr;23(8):2067-77.

Helwig et al. PC1/3 and PC2 gene expression and post-translational endoproteolytic pro-opiomelanocortin processing is regulated by photoperiod in the seasonal Siberian hamster (Phodopus sungorus).
J Neuroendocrinol. 2006 Jun;18(6):413-25.

Gotoh et al. Apolipoprotein A-IV interacts synergistically with melanocortins to reduce food intake.
Am J Physiol Regul Integr Comp Physiol. 2006 Jan;290(1):R202-7.

Wang et al. The regulation of glucose-excited neurons in the hypothalamic arcuate nucleus by glucose and feeding-relevant peptides.
Diabetes. 2004 Aug;53(8):1959-65.

Mousa et al. Subcellular pathways of beta-endorphin synthesis, processing, and release from immunocytes in inflammatory pain.
Endocrinology. 2004 Mar;145(3):1331-41.

Benoit et al. The catabolic action of insulin in the brain is mediated by melanocortins.
J Neurosci. 2002 Oct 15;22(20):9048-52.

Berman et al. Impaired prohormone convertases in Cpe(fat)/Cpe(fat) mice.
J Biol Chem. 2001 Jan 12;276(2):1466-73.

Zhang et al. Identification of a Novel Prohormone Sorting Signal-Binding Site on Carboxypeptidase E, a Regulated Secretory Pathway-Sorting Receptor
Mol Endocrinol. 1999 Apr;13(4):527-36.

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